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KMID : 1007520150240031035
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Food Science and Biotechnology
2015 Volume.24 No. 3 p.1035 ~ p.1040
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Effect of Low Frequency (20-35 kHz) Airborne Ultrasonication on Microbiological and Physicochemical Properties of Soybean Koji
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Byeon Ju-Yeong
Choi Eun-Ji
Kim Wang-June
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Abstract
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The effect of low frequency airborne ultrasound energy on microbiological and physicochemical properties of soybean koji during preparation was investigated. Following inoculation of steam-heated soybeans with spores of Aspergillus oryzae, koji samples were treated with airborne sonication at increasing frequencies of 20, 25, 30, and 35 kHz and incubated at 30oC for 5 days. Amylase and protease activities, the number of fungal spores, bacterial growth, the moisture content, pH, and the titratable acidity were analyzed during koji preparation. All frequencies of low ultrasound treatment tested enhanced enzyme activities. After continuous ultrasonication at 30 kHz, amylase, and protease activities were significantly (p<0.05) increased by 183 and 205%, respectively, relative to corresponding control groups.
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KEYWORD
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low frequency airborne ultrasonication, enzyme activity, soybean koji
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